 Solution

A stable multiplicative sensitivity factor $s_i$ at detector pixel $i$ changes both means to $N_i=s_i{N}_0$. The noise-free ratio is still one, so flat-field correction is not required merely to remove that fixed pattern from the mean ratio. This is the principal advantage of using two matched flats rather than the variance of a single image.

However, the photon shot noise is not cancelled. At high counts the conditional ratio variance is $2/(g{N}_i)$. Pooling detector pixels gives $E^2\simeq (2/g)⟨1/N_i⟩$, where brackets denote a spatial average. If the formula uses $\overline{N}=⟨N_i⟩$, it returnsThe inequality follows from the Jensen inequality for $1/x$ on positive signals. For small fractional response variation, the relative bias is approximately minus its squared coefficient of variation. A locally nearly uniform region or a fit using detector pixel-dependent variances avoids this bias from mixing the harmonic mean with the arithmetic mean. Stable response cancellation also presumes the conversion gain itself is uniform; gain variations, offset errors or changing sensitivity do not obey that simple cancellation. The fixed response pattern cancels in the ratio mean; unequal shot-noise levels can still bias a globally pooled gain estimate.