The Sec13-Sec31 complex forms the outer layer of a COPII coat. Its assemblies provide a scaffold around a budding carrier, while the inner Sec23-Sec24 complex couples coat formation to Sar1 GTPase and cargo selection. Reconstitution using purified coat components and defined liposomes demonstrated that this machinery can generate coated carriers without the full complexity of a cell. [ref]
COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes by Ken Matsuoka and others. 1998, purified Sar1p, Sec23-24p and Sec13-31p reconstitution
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