D. Sue Katz authored the American Society for Microbiology's 2008 explanation of the streak plate technique, a method for obtaining spatially separated bacterial colonies. The protocol connects a familiar laboratory pattern to its underlying purpose: dilution across a surface so that growth becomes distinguishable.[ref]
Successive spreading across an agar surface reduces the density of the inoculum in later regions. The resulting separated bacterial colonies can then supply material for investigating a pure culture.[ref]
The useful distinction is between a visible separation and proof of identity. A well-separated colony is an operational starting point for further characterization; its appearance alone does not establish every genetic or physiological property of the cells that produced it. This is a contribution to clear methodological teaching, rather than a claim that D. Sue Katz invented the historical procedure.
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