A granulosa-lutein cell is a granulosa cell that has undergone luteinization, acquiring the differentiated endocrine state associated with the corpus luteum. It can produce progesterone while retaining experimentally measurable regulation of other steroid pathways. The label identifies origin and cell state, rather than a separate oocyte or an entire ovarian follicle.
In a 1991 human-cell experiment, luteinized granulosa preparations were cultured for48hours with or without human chorionic gonadotropin or follicle-stimulating hormone. The investigators separately measured steroid production and aromatase activity. This demonstrated several regulated biochemical outputs in the luteinized preparation.[ref]
A later assay study addressed an important measurement problem: estradiol accumulation depends on available androgen substrate as well as aromatase. After a stimulation period, investigators washed the cultured granulosa-lutein cells and supplied testosterone for a separate short assay. Changing when substrate was supplied made the test of follicle-stimulating hormone responsiveness more interpretable.[ref]
A result from these cells should therefore identify the culture interval, stimulation and assay substrate. It cannot automatically describe the behavior of an unmodified granulosa cell at every stage of follicular development.

Ancestors (8)

  1. Granulosa cell
  2. Ovarian follicle
  3. Ovary (anatomy)
  4. Gonad
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