H. E. Alexander is the first author of a 1958 study showing that isolated poliovirus RNA could initiate infection in human cell culture. Cultures exposed to the preparation produced intact virus of the corresponding type. This supplied evidence that the RNA carried information needed for the infection process.[ref]
Infectivity of ribonucleic acid from poliovirus in human cell monolayers by H. E. Alexander and others. Abstract: isolated RNA infectivity and recovered virus; Journal of Experimental Medicine 108, 493–506, 1958
The ribonuclease control was particularly informative. Treatment destroyed the infectivity of the isolated RNA preparation but did not destroy infectivity of intact poliovirus. That contrast helped distinguish exposed infectious RNA from residual whole-virus contamination. The experiment concerned specially prepared material in culture; it did not show that unprotected RNA has all the transmission properties of an intact viral capsid-containing particle.[ref]
Infectivity of ribonucleic acid from poliovirus in human cell monolayers by H. E. Alexander and others. Abstract: ribonuclease and intact-virus controls, with dependence on assay conditions
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