The homotopy fiber of over consists of pairs where is a path from to . It replaces an ordinary fiber by a homotopy-invariant construction.
A principal fibration, in the homotopy-theoretic sense, is a homotopy pullback of a path-space fibration along a map . Its fibre is and acts on the fibre paths by concatenation, freely and transitively up to the relevant homotopies. Principal bundles for a topological group give this construction through its classifying space. When , the fibre is and the principal fibration is classified by , yielding a principal Eilenberg–MacLane fibration.
Let consist of paths starting at the chosen basepoint of . Endpoint evaluation is a fibration , and is contractible by shortening each path. Pulling it back along a map gives its homotopy fibre, after reversing the chosen path direction if needed. This makes an acyclic relative Sullivan algebra modelling useful for explicit rational fibre calculations.

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In algebraic topology, the concept of the homotopy fiber is a key tool used to study maps between topological spaces. It can be considered as a generalization of the notion of the fiber in the context of fibration, and it helps to understand the homotopical properties of the map in question.