J. C. Giddings is the coagulation researcher who coauthored the 1975 study of Factor V activation and prothrombin conversion with A. L. Bloom. The work investigated both assembly of reaction components and production of thrombin.[ref]
Gel chromatography separated the preparations, and specific antibodies helped identify components in the resulting fractions. Combined with kinetic measurements, these experiments supported a functional assembly involving phospholipids, Factor V, factor Xa and calcium ions.[ref]
Factor-V activation by thrombin and its role in prothrombin conversion by J. C. Giddings and A. L. Bloom. Abstract: Sephadex chromatography, immunological analysis and thrombin-generation tests
This illustrates two complementary questions: which molecules associate, and does their association support the expected reaction? Component identification addresses composition; an activity assay addresses function. Using both provides a stronger explanation than either separation or product measurement alone.
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