With zero fluid velocity and all mean orientations vertical, the steady cell conservation equation is
The plates are impermeable to cells, so the constant vertical flux is zero. Hence , giving . Determine by the prescribed depth average:
With and , the result is
The same normalized profile describes cell number density or cell volume fraction, because cell volume is constant. Its weak-stratification expansion is
which is depth-normalized at each displayed order and will be used in part (c). Upward swimming makes concentration larger near the upper plate, creating the density stratification responsible for bioconvection when cells are heavier than the fluid.

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