A Sholl profile records intersection counts as a function of distance from a chosen center. For a thin geometric tracing it is an exact counting function; a profile obtained from sampled images also depends on the software's sampling and segmentation conventions.
In an ideal planar model, let be a finite embedded rooted tree of line segments in the Euclidean plane with chosen root . Its Sholl profile counts distinct intersections:This is a geometric model for thin tracings; the software documentation separately addresses sampling binary images and handling image segmentation.[ref]
Sholl Analysis in the SNT documentation. Overview; Segmentation requirements; sampled profile metrics
For an original simple example, take three straight radial segments from the origin, pointing in different directions, with lengths 1, 2 and 4. For radii , the counts are respectively . The segment of length 1 contributes at but not beyond it; record the boundary convention when defining the count. No branch points are needed for this decreasing profile: different lengths of terminal segments already produce it.
The profile retains radial occupancy but discards the angles and the identities of the intersected paths. In particular, a Sholl profile does not determine rooted tree topology. Sampling only a finite list of radii loses still more information; it should not be silently equated with knowledge of the complete continuous-radius counting function.
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