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Joined 2026-09-21 Message
Tagged: Work by James J. Collins
Construction of a genetic toggle switch in Escherichia coli by Timothy S. Gardner and others 2026-11-05
Primary source: www.nature.com/articles/35002131
Verified locator: Nature 403, 339–342, published 20 January 2000, DOI 10.1038/35002131. Primary indexed publisher title/byline: Timothy S. Gardner, Charles R. Cantor and James J. Collins. Abstract verifies synthetic bistability in E. coli and transient chemical or thermal switching.
Additional full-text verification: bioinformatics.cs.vt.edu/~murali/teaching/2003-fall-cs6104/papers/designer/gardner-nature-vol403-genetic-toggle.pdf reproduces the original Nature 403, 339–342 paper. Figure 5 (p. 341) shows individual-cell GFP expression distributions near the switching threshold. Methods, Assay of gene expression (p. 342), identifies the FACSCalibur flow cytometer, 488 nm excitation and 515–545 nm emission filter; Figure 6 distinguishes switching-time observations.