 Cap analysis of gene expression

Cap Analysis of Gene Expression (CAGE) is a method used in molecular biology to study gene expression at the level of transcription. It primarily focuses on identifying and quantifying the transcription start sites (TSS) of genes, allowing researchers to understand where and how genes are being activated in different cell types or under various conditions. Here’s a brief overview of the CAGE process: 1. **Sample Preparation**: Cells or tissues of interest are collected, and RNA is extracted.