Ciro Santilli @cirosantilli 40

Super-resolution means resolution beyond the diffraction limit.

First you shine a lot of light which saturates most fluorophores, leaving very few active.

They you can observe fluorophores firing one by one. Their exact position is a bit stochastic and beyond the diffraction limit, but so long as there aren't to many in close proximity, you can wait for it to fire a bunch of times, and the center of the Gaussian is the actual location.

From this we see that super-resolution microscopy is basically a space-time tradeoff: the more time we wait, the better spacial resolution we get. But we can't do it if things are moving too fast in the sample.

Tradeoff with cryoEM: you get to see things moving in live cell. Electron microscopy fully kills cells, so you have no chance of seeing anything that moves ever.

Caveats:

Scientology music! So good.

It is not obvious why there have to be two sexes.

Sex itself is obvious: by mixing genes we increase variability.

But having two sexes rather than just being able to reproduce with anyone reduces the possible mating pool by half!

Likely a good replacement for Python. If the ecosystem gets there, Ciro Santilli would gladly use it more.

Mnemonic: sur means over. So we are going over the codomain, and covering it entirely.

In order to make websites efficient and portable, a lot of transpilation is needed.