Cleaved amplified polymorphic sequence

Cleaved Amplified Polymorphic Sequences (CAPS) are a molecular biology technique used for detecting polymorphisms (variations) in specific regions of DNA. This method combines PCR (Polymerase Chain Reaction) amplification with restriction enzyme digestion to differentiate between alleles based on the presence or absence of specific restriction sites. Here’s how CAPS works in general: 1. **PCR Amplification**: A target DNA region is amplified using PCR with primers that flank the polymorphic site.