Hybridization assay

A hybridization assay is a molecular biology technique used to detect and quantify specific nucleic acid sequences (DNA or RNA) within a sample. The fundamental principle of hybridization assays involves the formation of stable complexes between complementary nucleic acid strands, a process known as hybridization. These assays are pivotal in various applications, including gene expression analysis, genotyping, mutation detection, and the diagnosis of infectious diseases. **Key Components and Concepts:** 1.