Ligation-independent cloning

Ligation-independent cloning (LIC) is a molecular biology technique used to insert DNA fragments into vectors without the need for traditional DNA ligation processes. This method simplifies the cloning process and enhances efficiency, especially for the construction of recombinant DNA. ### Key Features of Ligation-Independent Cloning: 1. **End Modification**: The DNA fragments intended for cloning are modified at their ends to create complementary overhangs or "sticky ends." This is often achieved via polymerase-mediated addition of specific sequences.