Nuclear magnetic resonance spectroscopy of proteins

Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique used to determine the structure, dynamics, and interactions of proteins and other biomolecules at the atomic level. The method relies on the magnetic properties of certain atomic nuclei, primarily hydrogen (¹H), carbon (¹³C), nitrogen (¹⁵N), and phosphorus (³¹P). Here’s a more detailed overview of how NMR spectroscopy works in the context of proteins: ### Basic Principles of NMR 1.