Third-generation sequencing (TGS) refers to a set of advanced DNA sequencing technologies that enable the direct reading of nucleic acid sequences in a more rapid and cost-effective manner compared to earlier sequencing methods. Unlike first-generation (Sanger sequencing) and second-generation (next-generation sequencing, or NGS) techniques, third-generation sequencing allows for the sequencing of longer DNA fragments, which is particularly beneficial for resolving complex genomes, repetitive regions, and structural variants.
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