B. Passlick is the first-listed author of a 1989 study distinguishing a monocyte subpopulation in human peripheral blood. The study combined measurements of the surface markers CD14 and CD16 by flow cytometry. Cells expressing both markers were assigned to the monocyte lineage using their appearance, additional molecular markers and functional measurements, rather than relying on one marker alone.[ref]
Identification and characterization of a novel monocyte subpopulation in human peripheral blood by B. Passlick and others. Abstract, Blood 74 (1989), pp. 2527–2534; PMID 2478233.
A useful way to understand the experiment is to imagine plotting CD14 signal horizontally and CD16 signal vertically for individual cells. Two cells with similar CD14 signals can occupy different positions because their CD16 signals differ. A single-marker measurement would conceal that distinction. The paper also compared light scattering and cell size between the identified subset and the other monocytes, giving independent evidence that the two-marker pattern accompanied other cellular differences.[ref]
Identification and characterization of a novel monocyte subpopulation in human peripheral blood by B. Passlick and others. Abstract: CD14/CD16 coexpression, lineage tests, light scattering and cell-size comparison.
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