 Electrophoretic mobility shift assay

A binding assay that detects a change in electrophoretic mobility when a ligand associates with a labelled target, such as an RNA–protein complex. A concentration series and competition controls help distinguish specific binding from aggregation or non-specific retention.

An Electrophoretic Mobility Shift Assay (EMSA), also known as a gel shift assay, is a technique used to study protein-DNA or protein-RNA interactions. The primary purpose of EMSA is to assess the binding affinity of a protein to a specific nucleic acid sequence, which can provide insights into the regulation of gene expression and other biological processes.