 Fluorescence in situ hybridization

Detection of nucleic acid targets within cells or tissues using fluorescent probes. It connects sequence recognition with spatial position, while probe design and background affect which signal can be assigned to a target.

Fluorescence in situ hybridization (FISH) is a powerful cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. This method combines fluorescent labeling with hybridization techniques to facilitate the visualization of genetic material within the cell. Here’s how FISH works: 1. **Sample Preparation**: Cell samples are prepared, often from tissues or blood, and fixed onto a microscope slide.