In the GAL1 gene promoter system used in the yeast cross-species complementation experiment, the inducing sugar galactose promotes gene expression from the engineered construct, whereas glucose provides a noninducing comparison. The comparison concerns an imposed regulatory system, not the donor species’ native regulation. [ref]
Pathways Over Time Plasmids Engage Students in Functional Genomics Research by Clare O’Connor. O’Connor (2018), Plasmid features enable multiple student experiments and Complementation reveals conservation of function: GAL1 promoter
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