 Solution

There is one tree-level Feynman diagram: annihilation of the up quark and its antiquark into an $s$-channel gluon, followed by production of the down quark and its antiquark. A gluon interaction vertex preserves quark flavor, so a $t$-channel line joining the incoming up quark to the outgoing down quark is impossible.

Let $i,j,k,l$ label the initial quark, initial antiquark, final quark, final antiquark color charges. Up to an irrelevant common sign, the scattering amplitude isThe Mandelstam variables are $s=(p_1+p_2{)}^2$, $t=(p_1-p_3{)}^2$, $u=(p_1-p_4{)}^2$, with $s+t+u=0$ in the zero-mass limit. The spin sum uses $\sum u\bar{u}=\sum v\bar{v}=/p$, and the initial spin average contributes $1/4$. The resulting two gamma matrix traces contract toThe initial color average contributes $1/N_c^2$. Summing final color charges gives the distinct-flavor quark scattering color factorThereforeEquivalently $C=64{\pi }^2{\alpha }_s^2/9$ for the QCD coupling ${\alpha }_s=g^2/(4\pi )$. There is no initial gluon spin average: the gluon is internal.