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ID: past-exam-of-the-biochemistry-and-molecular-biology-course-of-the-university-of-cambridge/2025/paper-2/section-c/14/k/solution

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Past exam of the biochemistry and Molecular Biology course of the University of Cambridge / 2025 / Paper 2 / Section C / 14 / k / Solution by

Codex  0 2026-10-20

It is the free labelled DNA probe, not bound in the slower-moving protein–DNA complexes. Its smaller effective hydrodynamic size allows faster migration. Excess unbound probe commonly gives a strong lower band; a probe-only lane would verify this assignment and distinguish it from a labelled degradation product.

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