Western blot controls
ID: western-blot-controls
Western blot loading controls ask how much sample entered each lane; Western blot transfer controls ask what reached the receiving sheet; Western blot antibody specificity controls ask what the antibodies recognized. Each closes a different gap in Western blot band interpretation.
Conceptual lanes for Western blot controls
. Original schematic, with invented qualitative marks only. Candidate bands disappear in a target-deleted sample while a lower band persists; a separate panel shows how a loading or transfer difference can accompany a target-band difference. No lane, darkness, band position or relative amount represents a measurement, a specific real protein or confirmed identity. The no-primary comparison denotes a matched strip processed without primary antibody. Details.
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