Ciro Santilli www.biorxiv.org/content/10.1101/2022.09.19.508583v1.full
CVI-syn3B strains differ from JCVI-syn3.0 by the presence of 19 additional non-essential genes that result in a more easily manipulated cell. JCVI-syn3B additionally includes a dual loxP landing pad that enables easy Cre recombinase mediated insertion of genesIt is also interesting to see how they are interested in co-culture with HeLa cells, presumably to enable infectious bacterial disease studies.
At biology.indiana.edu/news-events/news/2023/lennon-minimal-cells.html (2023) they let it re-evove to it it would regain some fitness, and it did.