{c}
{title2=2022}

https://www.biorxiv.org/content/10.1101/2022.09.19.508583v1.full
> CVI-syn3B strains differ from <JCVI-syn3.0> by the presence of 19 additional non-essential genes that result in a more easily manipulated cell. JCVI-syn3B additionally includes a dual loxP landing pad that enables easy Cre recombinase mediated insertion of genes
It is also interesting to see how they are interested in co-culture with <HeLa> cells, presumably to enable infectious bacterial disease studies.

At https://biology.indiana.edu/news-events/news/2023/lennon-minimal-cells.html (2023) they let it re-evove to it it would regain some fitness, and it did.
