For real smooth coefficients with , the Rayleigh quotient has the displayed first variation. Its stationary points solve the Sturm-Liouville equation on an admissible class whose boundary variations make . Homogeneous Dirichlet boundary conditions do this by setting at the ends; homogeneous Neumann boundary conditions do it through . More general self-adjoint boundary conditions can require a boundary contribution to the quadratic form.
For an eigenfunction, the weaker identity suffices to give by integration by parts, but it does not by itself imply the condition on every variation. An explicit counterexample uses on and . This solves and has . Define a nearby family
It keeps , , , and , so for small . Nevertheless, has , , and , giving . Boundary-energy cancellation alone therefore cannot replace a specified variational domain.

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