Chris Anthony

Chris Anthony coauthored the 1964 study of a bacterial methanol dehydrogenase with L. J. Zatman. His own research account explicitly connects his name with the initial-only Anthony–Zatman publications.^[ref]

The experiment measured an optical response associated with electron transfer, using a dye-linked enzyme assay. The paper describes a Unicam SP.500 spectrophotometer and $10\mathrm{mm}$ cuvettes. Establishing the reaction product required separate chemical analysis rather than the optical signal alone.^[ref]

For an original illustration of the optical measurement, assume the Beer-Lambert lawwith absorbance $A=0.20$, liquid path $\ell =1.0\mathrm{cm}$ and a hypothetical molar coefficient $\epsilon =10^4\mathrm{L}\mathrm{mo}{\mathrm{l}}^{-1}\mathrm{c}{\mathrm{m}}^{-1}$. ThenA decrease of $0.05$ absorbance units would correspond to a $5\times 10^{-6}\mathrm{mol}{\mathrm{L}}^{-1}$ decrease of the absorbing species under those assumptions. It does not by itself give the amount of methanol consumed: the chemical identity of the absorber and the coupling stoichiometry must also be known. The coefficient and measurements are invented for this example.