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This produces a split-plot design: assign spray to whole orchards, with six sprayed and six unsprayed, then independently randomize ten trees to each pruning method inside every orchard. Orchards remain experimental units for spray; individual trees become experimental units for pruning. Pruning contrasts and the spray-by-pruning interaction term now lie in the within-block ANOVA stratum, while spray is tested between orchards.

The between-orchard ANOVA stratum has eleven statistical degrees of freedom, split into one for spray and ten for error. The within-orchard ANOVA stratum has 348, split into two for pruning, two for the interaction term and 344 for error. This pooling of within-orchard error is appropriate under the stated compound-symmetry covariance model; additional orchard-specific pruning variation would need its own variance component rather than this simplified error model.

The shared orchard effect cancels in a pruning difference within an orchard. Its variance is $2a/10$, so averaging across twelve orchards givesThe spray contrast still compares means of six orchards per group, each based on 30 trees, so its estimated variance remains $8/3$. For a difference of pruning differences between the two spray groups, each group's pruning difference has estimated variance $2\cdot 180/(10\cdot 6)=6$, and the resulting interaction contrast has estimated variance $12$, compared with $16$ in the original allocation.

Splitting pruning within orchards improves pruning and interaction precision without extra trees, and gives spray a less sparse error estimate; it does not reduce the spray contrast's variance. This option requires tree-level pruning to be practical without interference between neighboring trees. The numerical gains, like those in the other options, assume the current variance components remain applicable next year.

A replicate is an independently allocated instance of a treatment on an experimental unit. Independent replicates permit estimation of response variation; subsamples within one unit do not supply additional independent treatment replication.

Replication in experimental design assigns a treatment to multiple independently allocated experimental units. Repeated measurements on one unit can improve measurement precision without providing independent treatment replication.

A row-column design has two crossed partitions of experimental units into rows and columns. Equal treatment proportions in every row and every column make treatment contrasts orthogonal to both centered block spaces. A repeated three-treatment arrangement in a six-by-six array can give a balanced example.

For a vector of estimated treatment means $\hat{\mu }$, the variance of a treatment contrast with coefficient vector $c$ is $c^T\mathrm{Cov}(\hat{\mu })c$. Averaging subsamples from a common experimental unit does not make their shared variance component disappear.