An optical microscope whose microscope objective is designed to form a real image at a specified finite distance. The intended image distance and optical aberration corrections belong together; mechanical tube length is a mounting specification, not automatically the image distance measured from a principal plane.[ref]
Why can a small displacement produce a large image? For a converging thin lens, write with . Substitution into the thin lens equation gives
The similar triangles construction supplies the minus sign. The example has and hence . Letting decrease moves the real image farther away; it does not keep the microscope eyepiece in focus at a fixed position. Large transverse magnification therefore requires the correct conjugate geometry, not merely a short focal length.
An optical microscope whose microscope objective, with the specimen at its designed front focal plane, sends out a parallel bundle of light rays from each specimen point. Different specimen points generally give different bundle directions. A tube lens then forms the finite intermediate microscope image; the microscope eyepiece views it. This parallel region permits suitable optical accessories to be inserted. Finite-conjugate and infinity-corrected components must be matched to their intended system.[ref]
In the ideal paraxial approximation, an object height at objective focal length gives bundle slope . A tube lens of focal length maps that slope to , so
This is a two-assembly result. Substituting infinity into the single-objective transverse magnification would not describe the finite recorded image. Infinity correction concerns the region after the objective; relaxed-eye viewing concerns the region after the eyepiece. They are distinct conditions.