Micrococcal nuclease is a calcium-dependent nuclease capable of cleaving DNA and RNA. In chromatin analysis, accessible connecting DNA is often cut more readily than DNA protected by nucleosomes. The recovered fragment distribution can therefore report protection patterns, but sequence preference and digestion extent affect interpretation: a fragment boundary is not automatically an exact unperturbed cellular boundary. [ref]
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Micrococcal nuclease, also known as MCN or MNase, is an enzyme that is classified as a type of nuclease. It is produced by the bacteria *Staphylococcus aureus*. This enzyme is primarily known for its ability to cleave the phosphodiester bonds in nucleic acids, thereby breaking down DNA and RNA into smaller fragments.