For an independent second sample, let
Under , independence and the two chi-squared laws give the F-distribution
For the one-sided alternative , reject for large , using the upper critical value.
No values of the unknown means enter this statistic: centering by the sample means removes them. If both means are known, one may instead use sums about the known means; the corresponding degrees of freedom are and , rather than and . Thus knowledge of the means changes the degrees of freedom and critical value, while the variance-ratio principle is unchanged.

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