Choose homogeneous Dirichlet boundary conditions , and take a nonzero real admissible trial . Write the numerator as and denominator as . For an endpoint-zero variation , integration by parts gives
Thus the first variation of the Rayleigh quotient is
By the fundamental lemma of the calculus of variations, stationarity is equivalent to
Conversely, multiplying an eigenfunction equation by and integrating gives , so its first variation vanishes. This establishes the equivalence for the chosen Sturm-Liouville problem. Homogeneous Neumann boundary conditions, or a mixed Dirichlet-Neumann pair, also cancel the boundary term; general Robin boundary conditions require adding the corresponding boundary contribution to the numerator.

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