Use the standard regular Sturm-Liouville problem hypotheses: positive weight , real coefficients, and real self-adjoint boundary conditions. The positive weight and boundary-domain assumptions are implicit in the requested real orthonormal basis; real differential coefficients alone would not guarantee them for arbitrary complex boundary conditions.
Multiply the eigenfunction equation by and integrate. By self-adjointness, the number is real, whereasThe second integral is strictly positive, so . Because the coefficients and boundary conditions are real, the real and imaginary parts of any complex eigenfunction separately obey the same equation and conditions. At least one is nonzero, so a real eigenfunction can be chosen. More generally, each eigenspace has a real basis, which can be orthonormalized using the weighted inner product.
The orthogonality of Sturm-Liouville eigenfunctions, with normalization chosen to one, isFor the real choice, omit the complex conjugate.
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