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The cosmic microwave background is nearly isotropic thermal electromagnetic radiation. Its spectrum connects radio receiver measurements with the universe's thermal history: redshift preservation of a thermal photon spectrum cools a blackbody spectrum as space expands. The 1965 Princeton analysis described this relation.^[ref]

A radiometer cannot label a received photon as cosmic, atmospheric or instrumental. Its measured power combines the incoming radiation with the receiver response. The useful question is which comparisons and controls can isolate a sky contribution. This makes the Penzias-Wilson microwave background experiment an example of both observational cosmology and measurement calibration.

For a stable radiometer with affine output $S=g(T+T_r)$, let $g>0$ be the fixed response coefficient and $T_r$ the receiver noise temperature. Two reference loads with known equivalent input noise temperatures $T_c<T_h$ give outputs $S_c$ and $S_h$. Subtracting their outputs eliminates $T_r$; dividing source and reference differences eliminates $g$:This two-load radiometer calibration assumes unchanged response and receiver noise temperature between readings, with the loads and source referred to the same input. It also eliminates an additional constant output offset. Physical load temperatures must first be converted to the applicable equivalent input noise temperatures when the classical thermal approximation fails. The Penzias-Wilson microwave background experiment gives an original worked example.