Phosphorus-32 2026-11-05
Phosphorus-32 is a radioactive isotope of phosphorus with 15 protons and 17 neutrons. It undergoes beta-minus decay to sulfur-32, preserving the mass number while increasing the atomic number by one. The cited laboratory evaluation gives a half-life of .[ref]
Its radioactivity makes phosphorus-containing material experimentally traceable. In the 1952 Hershey-Chase experiment, phosphorus-32 labelled the DNA component of Bacteriophage T2. Mechanical agitation removed much less phosphorus label than sulfur label from infected bacteria, helping distinguish the material entering the cells from material remaining at the surface.[ref] The interpretation relies on the labelling and separation in this system; it is not a rule that all biological phosphorus belongs to DNA.
For a closed sample changing only by radioactive decay, . After two quoted half-lives, about 28.546 days, the radioactivity is one quarter of its initial value. A falling signal can therefore reflect decay even without loss of labelled material from a vessel. Movement of material, chemical processing and detector response require additional measurements when interpreting a real tracer experiment.
Sulfur-32 2026-11-05
Sulfur-32 is a stable isotope of sulfur whose atomic nucleus contains 16 protons and 16 neutrons. Its mass number is , the sum of those counts. The measured relative atomic mass is approximately ; a mass number is an integer count, whereas a relative atomic mass is a mass ratio.[ref]
The same NIST table gives a representative isotopic abundance of , or , making sulfur-32 the predominant natural sulfur isotope. This is a representative composition, not an immutable percentage in every sample. Using the listed sulfur-34 fraction , the corresponding isotope ratio is : about 4.47 sulfur-34 atoms per 100 sulfur-32 atoms.[ref] An isotope ratio and a percentage of all sulfur atoms have different denominators.
Sulfur-32 is also the daughter of phosphorus-32 beta-minus decay. One nuclear neutron becomes a proton, changing to while retaining mass number .[ref] This connects a radioactive tracer to a stable daughter without turning their chemical identities into the same thing.