Phosphorus-32 (${}^{32}\mathrm{P}$)

Phosphorus-32 is a radioactive isotope of phosphorus with 15 protons and 17 neutrons. It undergoes beta-minus decay to sulfur-32, preserving the mass number while increasing the atomic number by one. The cited laboratory evaluation gives a half-life of $14.273(7)\mathrm{d}$.^[ref]

Its radioactivity makes phosphorus-containing material experimentally traceable. In the 1952 Hershey-Chase experiment, phosphorus-32 labelled the DNA component of Bacteriophage T2. Mechanical agitation removed much less phosphorus label than sulfur label from infected bacteria, helping distinguish the material entering the cells from material remaining at the surface.^[ref] The interpretation relies on the labelling and separation in this system; it is not a rule that all biological phosphorus belongs to DNA.

For a closed sample changing only by radioactive decay, $A(t)/A(0)=2^{-t/T_{1/2}}$. After two quoted half-lives, about 28.546 days, the radioactivity is one quarter of its initial value. A falling signal can therefore reflect decay even without loss of labelled material from a vessel. Movement of material, chemical processing and detector response require additional measurements when interpreting a real tracer experiment.