Electrophoretic separation in a polyacrylamide matrix. Native conditions can preserve molecular complexes, whereas denaturing conditions address different separation goals.
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Polyacrylamide gel electrophoresis (PAGE) is a laboratory technique used to separate macromolecules, primarily proteins and nucleic acids, based on their size and charge. The method involves the use of a polyacrylamide gel, which serves as a medium through which the molecules can migrate when an electric field is applied.