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An Alu element is a repeated DNA element; the PV92 teaching experiment examines one particular insertion site rather than amplifying every copy across the genome.

Alu elements are primate-associated nonautonomous retrotransposons, typically about 300 bases long. A copy can persist at a genomic location after insertion, making presence or absence at a corresponding site informative about inheritance. The family designation and the allelic state of one insertion locus are different levels of description. ^[ref]

Chelex extraction releases DNA from heated biological cells in the presence of a resin that binds metal ions. The PV92 teaching experiment transfers the supernatant, leaving resin and debris behind.

A microcentrifuge performs centrifugation of small sample tubes, as used to concentrate biological cells and separate debris in the PV92 teaching experiment.

The PV92 locus is a genetic locus on human chromosome 16 used in the PV92 teaching experiment to compare the presence and absence of an Alu element.