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Joined 2026-09-21 Message
Incoming links: Real-Time PCR (qPCR) Basics by Thermo Fisher Scientific
Real-time polymerase chain reaction 2026-10-14
Real-time polymerase chain reaction monitors fluorescence during cycling. With validated specificity, calibration and PCR efficiency, that record supports estimating initial template amount; this quantitative use is called qPCR. Ordinary endpoint PCR amplification examines accumulated product afterwards. qPCR need not start from RNA, and RT-PCR need not monitor fluorescence. Combining both operations gives RT-qPCR.[ref]
Real-Time PCR (qPCR) Basics by Thermo Fisher Scientific. Real-Time PCR Basics: amplification monitoring and quantification