A steady-current image is not supported by the electrical connection drawn. tunnelling atomic force microscopy requires a conducting AFM probe, an applied bias voltage and a continuous return path from the contacted region to the current amplifier. graphene is conducting, but a flake on insulating glass is not electrically grounded merely because the other side or edge of the glass is earthed. Charging transients or displacement current are not its steady conductivity. Add a metal contact actually touching the graphene before using its current contrast; ordinary force-based surface topography can still be recorded without that contact. The manufacturer's nanostructure measurements explicitly distinguish objects connected to conducting pads from unconnected particles on the same insulating substrate.[ref]
quantitative nanomechanical mapping can image the flake without an electrical return path. Repeated AFM force curves provide surface topography, adhesion and deformation or effective elastic modulus channels. The step and differences in tip–surface interaction can distinguish graphene from glass. For an atomically thin supported sheet the contact response is strongly substrate dependent, so fitting a three-dimensional half-space contact model does not directly measure the intrinsic in-plane Young's modulus of free graphene. Calibrate the cantilever spring constant, deflection sensitivity and tip radius, use small loads, and compare bare substrate. The manufacturer's description specifies the force-curve origin of the mechanical channels.[ref]