Microscopy connectome extraction by Ciro Santilli 34 Updated +Created
This is the most plausible way of obtaining a full connectome looking from 2020 forward. Then you'd observe the slices with an electron microscope + appropriate Staining. Superintelligence by Nick Bostrom (2014) really opened Ciro Santilli's eyes to this possibility.
Once this is done for a human, it will be one of the greatest milestone of humanities, coparable perhaps to the Human Genome Project. BUt of course, privacy issues are incrediby pressing in this case, even more than in the human genome project, as we would essentially be able to read the brain of the person after their death.
As of 2022, the Drosophila connectome had been almost fully extracted.
This is also a possible path towards post-mortem brain reading.
Figure 1. Source. Unconfirmed, but looks like the type of frozen brain where a Microtome would be used.