Ciro Santilli @cirosantilli  40

No support:Example with raw examples under nodejs/sequelize/raw/many_to_many.js

The first/only way Ciro could find was with ampy: stackoverflow.com/questions/74150782/how-to-run-a-micropython-host-script-file-on-the-raspbery-pi-pico-from-the-host/74150783#74150783 That just worked and it worked perfectly!

TODO: possible with rshell?

Examples at: Raspberry Pi Pico W MicroPython example.

Examples at: Raspberry Pi Pico W MicroPython example.

An upstream repo at: github.com/raspberrypi/pico-micropython-examples

Some generic Micropython examples most of which work on this board can be found at: Section "MicroPython example".

Pico W specific examples are under: rpi-pico-w/upython.

The examples can be run as described at Program Raspberry Pi Pico W with MicroPython.

One cool thing we did in this procedure was to use magnetic separation with magnetic beads to further concentrate the DNA: Figure 1. "GE MagRack 6 pipetting.".

The beads are coated to stick to the DNA, which allows us to easily extract the DNA from the rest of the solution. This is cool, but bio people are borderline obsessed by those beads! Go figure!

Then we prepared the DNA for sequencing with the Oxford Nanopore specific part: Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit.

Here some of the steps required a bit more of vortexing for mixing the reagents, and for this we used the VELP Scientifica WIZARD IR Infrared Vortex Mixer which appears to be quicker to use and not as strong as the Vortex Genie 2 previously used to break up the cells:

After all that was done, the DNA of the several 400 ml water bottles and hours of hard purification labour were contained in one single Eppendorf!