Overlap extension polymerase chain reaction

Overlap Extension Polymerase Chain Reaction (OE-PCR) is a molecular biology technique used to generate specific DNA fragments or constructs, often for the purpose of cloning, mutagenesis, or creating fusion genes. This method leverages the principles of standard PCR but incorporates overlapping regions between primer pairs, allowing for the precise assembly of multiple DNA fragments. ### Key Features of OE-PCR: 1. **Design of Primers**: In OE-PCR, primers are designed to have overlapping sequences.