A restriction digest is a molecular biology technique used to analyze DNA by cutting it into smaller fragments using restriction enzymes, also known as restriction endonucleases. These enzymes recognize and cleave specific sequences of nucleotides in the DNA, typically at palindromic sites, which are the same when read in the 5' to 3' direction on both strands.
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A restriction digest is cleavage of DNA using a restriction enzyme. Complete cutting at all specified sites and partial cutting can give different fragment patterns.