Subcloning is a molecular biology technique that involves the transfer of a specific DNA fragment (such as a gene, promoter, or regulatory element) from one plasmid or vector to another. This process is used to create a new DNA construct with desired features, often for research, genetic engineering, or therapeutic applications. Key steps in subcloning typically include: 1. **Restriction Digestion**: The original DNA fragment and the new vector are cut with specific restriction enzymes to create compatible ends.
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