A. Lambert is the first author of a 2000 study improving measurements of follicle-stimulating hormone effects on aromatase in human granulosa-lutein cells. These cells, collected during in-vitro fertilization procedures, convert testosterone to estradiol through aromatase. The study addressed a measurement problem: hormone output during a long incubation depends both on the amount of active enzyme and on how much substrate remains.[ref]
Improved FSH sensitisation and aromatase assay in human granulosa-lutein cells by A. Lambert and others. Abstract: human follicular aspirates and interpretation of prolonged testosterone exposure
The researchers separated the stages. They first preincubated the granulosa-lutein cells, stimulated them with follicle-stimulating hormone for 24 hours, washed them, and then supplied testosterone for a two-hour assay. The short challenge limited substrate depletion and isolated the later measurement from the earlier stimulation. After preincubation, the observed stimulation was three- to fourteen-fold over basal activity, depending on the preparation. This illustrates why an enzyme assay needs control of both biological regulation and reaction conditions.[ref]
Improved FSH sensitisation and aromatase assay in human granulosa-lutein cells by A. Lambert and others. Abstract: 0/24/48-hour preincubation, 24-hour stimulation and two-hour testosterone challenge
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