G. Gamba is the first author of a 1994 study identifying two related salt-transport proteins in the mammalian kidney. The team cloned the corresponding DNA sequences and tested the expressed proteins in Xenopus laevis oocytes. One transported sodium, potassium and chloride; the other transported sodium and chloride without the same potassium requirement.[ref]
Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney by G. Gamba and others. Abstract: cloned cDNAs and functional expression; Journal of Biological Chemistry 269, 17713–17722, 1994
The transporters also differed in their responses to inhibitors, including bumetanide. Combining sequence comparison, transport assays and localization within the kidney established a family resemblance without assuming identical function. This is an important experimental distinction: a similar protein primary structure suggests a relationship, while controlled measurements of ion movement test what each membrane transport protein actually does.[ref]
Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney by G. Gamba and others. Abstract: sequence similarity, differing ion requirements and inhibitor sensitivity, and intrarenal expression
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