N. Kitamura is the first-listed author of the 1981 study that determined the primary sequence and organization of poliovirus RNA and related it to the expressed polypeptides. The original byline is retained here without inferring a longer personal identity.[ref]
The study reported a 7,433-nucleotide RNA sequence and an open reading frame spanning 2,207 consecutive triplets. Sequence analysis mapped twelve viral polypeptides to products of proteolysis of one encoded polyprotein. Connecting nucleotide order with the isolated protein products supplied evidence about gene expression and processing, rather than simply listing the genome's chemical composition.[ref]
Primary structure, gene organization and polypeptide expression of poliovirus RNA by N. Kitamura and others. Abstract: RNA length, coding frame and mapped cleavage products
An original consistency calculation gives nucleotide positions in those triplets, about of the reported sequence. This explains the stated scale of the long reading frame. It does not by itself locate a cleavage site: mapping the protein products requires additional sequence evidence.
Kitamura's contribution in this work links RNA sequence, an open reading frame and experimentally identified polyprotein products. Genome organization and subsequent protein processing answer related but distinct questions.
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