 Solution

False in general. Take $A=\mathrm{diag}(1,6,6)$ and $B=\mathrm{diag}(2,2,9)$. Both have trace $13$ and determinant $36$, but their eigenvalue multisets are different. For $n>3$, append equal identity blocks to preserve this counterexample. In dimensions one and two the assertion is true: in dimension two the characteristic polynomial is $t^2-(\mathrm{tr}A)t+\det A$, whose roots, with multiplicity, are determined by those two quantities.